Department of Development and Regenerative Biology, Icahn School of Medicine at Mount Sinai, NY, USA) with transgenic Alb-Cre recombinase (purchased from Jackson Laboratory; (C57BL/6 x DBA)F2 background; strain number 003574), respectively. The littermates that do not express Alb-Cre recombinase were used as control mice (Fgf21fl/fl and Fr1fl/fl, respectively). The 6th generation of Fgf21alb-cre mice and Fr1alb-cre mice was used for the experiments. All mice were housed in temperature-controlled animal facilities with 12-hour light/12-hour dark cycles and were permitted consumption of tap water and Purina standard chow ad libitum until being fed the experimental diets. The mice received humane care, and experiments were carried out according to the criteria outlined in the Guide for the Care and Use of Laboratory Animals and with approval of the Mount Sinai Animal Care and Use Committee. Nrf2 knockout (Nrf2−/−) mice were fed ethanol in the lab of Dr. Arndt Vogel in the Department of Hepatology, Gastroenterology and Endocrinology, Hannover Medical School, Hannover, Germany as described in (Lamlé et al., 2008). The liver samples from Nrf2−/− mice were generously provided by Dr. Vogel.
