(Teo et al., 2011) revealed significant up- or down-regulation of 9 out of these 12 markers with EtOH treatment (Fig. S3C). Based on the above data and the profound effects of EtOH exposure during the blastocyst stage on subsequent embryo development and viability (Padmanabhan and Hameed, 1988), we next focused on the effect of EtOH on differentiation-related gene expression in hESCs. To identify specific genes that may be affected by EtOH during differentiation of hESCs, genes that are differentially regulated during EB formation were compared to genes differentially regulated upon EtOH treatment in undifferentiated hESCs (Fig. 5). From this analysis we have identified a subset of genes significantly altered by EtOH treatment that are potentially involved in differentiation of hESCs into EBs (Orange box, Fig. 5). Due to the figure size limitations, only top 10 genes are illustrated in each panel. The complete list of genes for each panel is shown in the Supplemental Table 1.
