The mRNA co-expression modules significantly correlated with AD were assessed for enrichment of cell-type specific marker genes. All six modules showed cell-specific enrichment after correction for multiple testing (Table 1). Similar to other alcohol related studies in Prefrontal Cortex and Ventral Tegmental Area, our analysis revealed that alcohol has discrete effects on different cell types [40]. For example, the neuronal expression-associated modules, M yellow and M turquoise, were negatively correlated with AD status, while the glial and microglial-associated modules, M green, M grey60, and M salmon, were positively correlated with AD. M pink was also positively correlated with AD; however, this module was significantly enriched for astrocyte cell specific gene expression only.
