dimethyl sulfoxide was added, and the mixture was printed on CMT-GAPS-coated slides (Corning, NY) using an OmniGrid MicroArrayer OGR-03 (Genomic Solutions, San Carlos, CA). Each cDNA clone was printed in duplicate on each slide. After arraying, slides were UV-linked, washed in 3× SSC twice and in H2O once, then incubated with I-Block at 60°C for 30 min, followed by washing and storage in an airtight desiccator until use.
