Informative heterozygous SNPs in the mRNA region of selected X chromosome and imprinted genes were identified using microarray SNP genotyping data (Laurent et al., 2011). Total RNA was collected as above and converted to cDNA (Quantitect Reverse Transcription Kit, Qiagen) according to the manufacturer’s protocol. 40 ng of cDNA was then used as input for a Taqman qPCR SNP genotyping array selected to determine allelic expression. Quantitative expression data were acquired and analyzed using a CFX-96 Real-Time PCR Detection System (BIORAD) using the SNP genotyping taqman probes DDX26B (C_16188987_10), MAMLD1 (C_15867801_10), RPGR (C_11874860_10), SLC25A43 (C_25953804_20), USP51 (C_27476233_10), PEG10 (C_25805777_10) and PEG3 (C_25643544_10).
