three screws was a silver 34 gauge insulated wire (Cooner wire, Chatsworth, CA), to which a pin contact (amphenol) was crimped. The three pins were adhered together with dental cement at an angle perpendicular to the saggital suture and shrink wrap was applied around each pin. Additional dental cement was applied outside the shrink wrap to secure it to the skull. Mice were then returned to their home cage which was housed within a recording chamber and allowed 3-9 days to recover before recording procedures.
