in duplicate, obtaining more than 30 million pairs of reads per sample, resulting in approximately 24,000-fold coverage depth after mapping (Tables S3 and S4 in Additional file 2). Consistent with our previous report [13], the coverage distribution is uniform (82% of amplicons between 0.5- and 2-fold of the mean coverage) and reproducible between samples and across sequencing runs (Figure S3 in Additional file 1 and Table S4 in Additional file 2).
