5 ng of genomic DNA isolated from undifferentiated ReN-m and ReN-mAP cells was PCR amplified using TaqMan(r) probes for the two APOE SNP markers (rs429358, Cat. No.:C_3084793_20 and rs7412, Cat. No.:C_904973_10) using TaqMan(r) Universal Master Mix II (Life Technologies). DNA samples of known APOE-e4 genotypes (in duplicate) were used as controls to obtain the genotype clusters, and all the samples were PCR amplified on the CFX384 thermal cycler (Bio-Rad, Hercules CA, USA).
