Chunk #51 — Methods — Transient transfections and protein-RNA purifications

Source

DALRD3 encodes a protein mutated in epileptic encephalopathy that targets arginine tRNAs for 3-methylcytosine modification.

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FLAG- purifications for the DALRD3 truncations followed the same protein purification protocol outlined above, however, we used Anti-DYKDDDDK Magnetic Beads (Clontech) as our purification resin. These purifications than underwent the TRIzol-RNA extraction procedure.