FSCV is a differential technique and measures changes in analyte concentration. To remove background and charging currents, ten cyclic voltammograms were recorded before an event (VTA stimulation or behavioral response), averaged and subtracted from all the cyclic voltammograms collected in the data file. The voltammograms were then plotted with the abscissa as acquisition time of the cyclic voltammogram, the ordinate as the applied potential, and the current in false color (Michael et al., 1998).
