Chunk #9 — 2. Methods — 2.5 RNA preparation and microarray hybridization

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PPAR agonists regulate brain gene expression: relationship to their effects on ethanol consumption.

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We have used the Illumina platform frequently in the past with repeated validations using independent qRT-PCR experiments. Correspondence between the microarray and RT-PCR results exceeds 80% 17, 41, 42.