Across discrete stimulus intensities, we found that the charge carried by the GYKI-resistant, KAR-mediated synaptic responses were significantly increased in CIE neurons, but not in cells recorded from CON and WD slices (Fig 2B & C). Likewise, the amplitude of the GYKI-resistant KAR synaptic response was significantly greater in CIE neurons than in either CON or WD neurons at both the 25μA stimulus (CIE, 27.3±7.7pA, n=7; CON, 10.1±2.2pA, n=7; WD, 11.2±1.5 pA, n=8; P<0.05 with One-way ANOVA and Newman-Kuels post-test) and the 35μA stimulus intensity (CIE, 56.4±12.2pA; CON, 23.0±4.5pA; WD, 27.55±5.64pA; P<0.05 with One-way ANOVA and Newman-Kuels post-test). These data suggest that the KA receptor contribution to BLA synaptic responses following EC electrical stimulation is increased during CIE relative to CON and returns to CON levels during WD.
