Chunk #40 — Methods — Cytokine array screen

Source

Neurotoxic reactive astrocytes are induced by activated microglia.

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yes

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Conditioned media from immunopanned purified and cultured microglia grown in non-reactive, or LPS-induced reactive state was collected as above and 100 μg of total protein was incubated with a Rat Cytokine Antibody Array Kit (R&D Systems, ARY008) according to manufacturer protocols.