The release of LDH in the culture supernatant of astrocytes was measured using the colorimetric Cyto-Tox 96 Non-radioactive Assay (Promega) following the manufacturer’s instructions. This assay is a coupled enzymatic assay, which results in the conversion of a tetrazolium salt into a formazan product which, in turn, is proportional to the number of lysed cells. We used triton X-100 as a positive control. The LDH value was expressed as the percentage of total LDH activity, according to the following equation: %LDH release rate = 100 × [(Experimental - Effector Spontaneous - Target Spontaneous)/(Target Maximum - Target Spontaneous)].
