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Chunk #36 — EXPERIMENTAL PROCEDURES — Sample collection and culture conditions

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Recurrent variations in DNA methylation in human pluripotent stem cells and their differentiated derivatives.
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All samples were either cultured in house or obtained from collaborators (for sample details, see Table S1). Plat-A Packaging cells (Cell Biolabs, Inc.) were maintained according to the manufacturer’s instructions. Human dermal fibroblasts (HDFs, ScienCell Research Laboratories) were cultured in Dulbecco’s modified eagle medium, 2mM GlutaMax, 10% fetal bovine serum and 0.1 mM non-essential amino acids (Life Technologies, Inc.). Culture conditions for hPSCs are listed in Table S1.