After seeding and transfection, cells were incubated for 48 h before freezing at −80 degrees overnight. To read luminescent activity, plates were thawed for 45 min at room temperature. Then 100 µL of Steady-Glo reagent (Promega #E2520) was added and incubated for 30 min at room temperature. Then luminescence was read for 2 s per well on a 384-well compatible plate luminometer (Molecular Devices LMax384).
