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Chunk #11 — Materials and Methods — Marker Selection and Genotyping

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Variants in nicotinic acetylcholine receptors alpha5 and alpha3 increase risks to nicotine dependence.
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were measured by the Analyst Fluorescence Plate Reader (LJL Biosytems, Sunnyvale, CA). Based on the ratio of fluorescence intensities, genotypes were scored by a Euclidean clustering algorithm developed in our laboratory [van den Oord et al., [2003]]. After genotyping, genotypes were cleaned and checked with the HaploView program [Barrett et al., [2005]] for deviation from Hardy-Weinberg Equilibrium. Details of marker characteristics were shown in Table II. Of the seven markers typed, rs6495038 and rs8192475 had mild deviations in the VAANX-ND and VAFTND samples, respectively (Table II).