Analysis of copurifying RNAs revealed the co-purification of tRNA with both METTL2A and 2B (Fig. 3b, lanes 8–9 and 11–12). Moreover, there was an increase in the amount of tRNAs co-purifying with METTL2A or 2B when each was co-expressed with DALRD3 (Fig. 3b, compare lanes 8 and 9 with 11 and 12). The increase in co-purifying tRNAs with METTL2A/B when co-expressed with DALRD3 is not due to an increase in METTL2A/B expression or purification since there was actually less METTL2A/B expressed and purified in the presence of DALRD3 (Fig. 3a, lanes 11 and 12).
