Chunk #6 — Results — 30-Day Exposure of hiPSC-Derived NPCs to Commercial Astrocyte Media Is Sufficient to Robustly Generate hiPSC-Astrocytes

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An Efficient Platform for Astrocyte Differentiation from Human Induced Pluripotent Stem Cells.

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S1B–S1D), ScienCell medium was selected owing to its lower cost and relative simplicity.Table 1Screening Conditions for Astrocyte DifferentiationIDReferenceBasal MediumSupplement 1Supplement 2Growth Factors1Brennand and Gage (2011)DMEM/F12N2, B27GlutaMAX + sodium bicarbonate + sodium pyruvateBDNFGDNFcAMPAA2ScienCell 1801ND (AM)AGS2% FBS3Thermo Fisher A1261301DMEMN2GlutaMAX + D-glucose + sodium pyruvate10% FBS4Lonza CC-3186ND–L-glutamineEGF3% FBSinsulin + AA5C.M.K., unpublished dataneural basal glutamine2× N2FGF2 + CNTF6modified from C.M.K., unpublished dataneural basal glutamineN2, B27GlutaMAX, NEAA, bMEFGF2 + CNTF7Krencik et al. (2011)DMEM/F12N2, B27GlutaMAX, NEAA, bMEFGF2 + CNTF8Jiang et al. (2013)DMEM/F12N2, B27GlutaMAX, NEAA, bMEFGF2 + CNTFBMP49reduction of % FBS from no. 10DMEM/F12N2, B27GlutaMAX, NEAA, bMEFGF2 + CNTFBMP43% FBS10Roybon et al. (2013)DMEM/F12N2, B27GlutaMAX, NEAA, bMEFGF2 + CNTFBMP410% FBS11Shaltouki et al. (2013)DMEM/F12N2, B27GlutaMAX, NEAA, bMEFGF2 + CNTFBMP43% FBSneuregulin12adding insulin + AA to no. 9DMEM/F12N2, B27GlutaMAX, NEAA, bMEFGF2 + CNTFBMP43% FBSinsulin + AAThe 11 screened conditions of astrocyte differentiation from hiPSCs, including 3 commercially available astrocyte media (nos. 2–4) and 8 conditions modified from published protocols (nos. 5–12), with a forebrain neuron differentiation protocol (no. 1). NEAA, non-essential amino acid; bME, 2-mercaptoethanol; AA, ascorbic acid; ND, non-disclosure; AM, astrocyte medium; AGS, astrocyte growth supplement.