Adult male C57BL/6 mice, purchased from Jackson Laboratories (Bar Harbor, ME), were used in this study. Mice were individually housed under a 12-hr light/dark cycle (lights on at 4:00 AM) in a temperature- and humidity controlled- animal facility. The animals had free access to food (Teklad rodent diet) and water throughout the experiment. The study began after an acclimation period (one week) and mice were monitored daily by the animal facilities staff and the research technicians.
