GCaMP6 performance was compared to other sensors in several ways. The fraction of responding neurons detected with GCaMP6s was 3-fold higher than for GCaMP5G (5-fold higher than GCaMP3) (Fig. 2e). Notably, the fractions of active neurons detected with GCaMP6s and GCaMP6m were also significantly higher than for OGB1-AM (Fig. 2e, f, p<0.01, Wilcoxon rank sum test). GCaMP6 sensors thus reveal neuronal dynamics that were previously undetectable with protein sensors.
