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Chunk #5 — Methods — Subjects

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A protocadherin gene cluster regulatory variant is associated with nicotine withdrawal and the urge to smoke.
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The NicW diagnosis was based on DSM-IV, which requires that four NicW symptoms be experienced within the first 24 hours after either quitting or smoking less than usual, or smoking (or using other nicotine containing products) to avoid problems caused by quitting or smoking less than usual. The NicW symptoms included: 1) Were you irritable, angry, or frustrated? 2) Were you nervous or anxious? 3) Were you restless? 4) Did you have trouble concentrating? 5) Did your heart slow down? 6) Did you feel down or depressed? 7) Did you have such a strong desire for cigarettes that you couldn’t think of anything else? 8) Did your appetite increase or did you gain weight? 9) Did you have trouble sleeping? Table 1 shows the number of subjects and NicW rates for each study group and demographic and smoking-related characteristics.