To have a visual representation of these distributions, select the checkboxes of the categories of interest, and click on the ‘Graph selected categories’ button. The graph will be displayed in a new window (Figure 8B). The x-axis is your uploaded value. The y-axis is the cumulative fraction. The blue curve is the overall distribution for all genes. The red curve is the selected functional category. In this case, it is PDGF signaling pathway. If you look at the data point x = −2.5, y is 0.3 for the red curve and 0.1 for the blue curve. This means that 30% of your uploaded genes have a value of −0.25 or smaller, but only 10% of the overall genes have a value of −0.25 or smaller. In other words, it shows that the distribution of the category tends to be smaller than the overall distribution. We find that this is critical for interpreting any deviation between the functional category distribution and the overall distribution.
