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Chunk #5 — Methods — Subjects

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Association between KCNJ6 (GIRK2) gene polymorphisms and postoperative analgesic requirements after major abdominal surgery.
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Subjects for the resequencing of the KCNJ6 gene were recruited from the Kanto area in Japan. A total of 48 unrelated healthy subjects were used in the study so that polymorphisms with allele frequency more than approximately 1% could be detected. The oral mucosa of the participants was collected for gene analysis.