All cultured cell lines were maintained in a humidified 37°C incubator with 5% CO2. HEK 293T, HepG2, and NIH 3T3 cells were all obtained directly from American Type Culture Collection and were cultured in high-glucose DMEM (Thermo Fisher Scientific) supplemented with 10% Fetal Bovine Serum (FBS; Thermo Fisher Scientific) and 1% penicillin/streptomycin (P/S). HUES 8 cells (directly obtained from the Harvard Stem Cell Institute iPS Core Facility), H7 cells (directly obtained from the WiCell Research Institute), DiPS 1016 SevA cells (directly obtained from the Harvard Stem Cell Institute iPS Core Facility), and all of the iPSC lines generated for this study (see below) were grown under feeder-free conditions on Geltrex (Thermo Fisher Scientific)-coated plates in chemically defined mTeSR1 medium (STEMCELL Technologies) supplemented with 1% P/S and 5 μg/mL Plasmocin (InvivoGen). Medium was changed every 24 hours. The cell lines were routinely tested and confirmed to be negative for mycoplasma contamination, as described below.
