In this study, we described a method by which mouse or human Ngn2 transduction can rapidly induce hiPSC-derived NPCs into functional excitatory neurons. We have now applied this method to hiPSC derived NPCs from more than twenty individuals (cases and controls). Although we have observed some variation in both the efficiency of lentiviral transduction and Ngn2-induction between individuals, we believe that this is a scalable method that can be routinely applied to a robustly proliferative starting population. We hope that this will be a useful platform for modeling neuropsychiatric disorders with patient-derived hiPSCs and for developing high throughput drug screens to identify novel therapeutics for the treatment of these diseases.
