n = 28; D24 39.99 ± 10.53 pA*ms, n = 25; D38 52.77 ± 25.81 pA*ms, n = 25). The glutamate receptor antagonist KyA (3 mM) blocked the sEPSC (data not shown). Spontaneous inhibitory postsynaptic current (sIPSC) could be recorded after 1 week of differentiation. Both the amplitudes (Fig. 6F, D4 0 ± 0 pA, n = 18; D7 2.4 ± 1.3 pA, n = 18; D24 22.7 ± 3.4 pA, n = 25; D38 35.8 ± 5.1 pA, n = 25) and frequencies (Fig. 6G, D4 0 ± 0 Hz, n = 18; D7 0.003 ± 0.002 Hz, n = 18; D24 0.12 ± 0.036 Hz, n = 25; D38 0.296 ± 0.064 Hz, n = 25) of the sIPSCs increased with time. Also, the area of the sIPSCs increased over time (Fig. 6H, D4 0 ± 0 pA*ms, n = 18; D7 97.4 ± 53 pA*ms, n = 18; D24 1214.1 ± 152.5 pA*ms, n = 25; D381364 ± 156.6 pA*ms, n = 25). The GABA receptor antagonist SR95531 (10 µM) completely blocked sIPSCs (data not shown).
