The Sequenom protocol validated 12 genes of the 26 predicted to be differentially methylated by 5mC-immunoprecipitation and microarray. Overall, verified hypermethylation was found in genes such as insulin growth factor (Igf2), SRY-box containing gene 7(Sox7), and cut-like 2 (Cux2) that are mediators cell cycle progression, neuronal regulation, and stem cell patterning. From this study, the authors conclude that ethanol alters the DNA methylation program of stem cell and differentiation qualities of NSCs.
