These results suggest that FOXG1 is involved, at least in part, in causing the overproduction of neurons of the GABAergic lineage found in ASD-derived organoids. FOXG1 RNAi had no or minor effects on the transcript/protein expression levels of dorsal forebrain markers (such as PAX6) (Figure 6 B–F, J, L, M), or on TFs directing cortical excitatory neuron differentiation (such as TBR1) (Figure 6M).
