A novel sliding-window method, written in PERL, was used to identify clusters of neighboring CpGs exhibiting concordant changes in methylation associated with cross-generational THC exposure (termed differentially methylated regions, DMRs; Supplementary Figure S2). DMRs were defined as regions of the genome containing at least three neighboring CpGs within a 500-bp interval; we required the presence of a minimum of three statistically significant CpGs (q<0.01) with a concordant (either hypo- or hypermethylated) mean methylation difference >2% between THC and VEH groups, representing at least 50% of CpGs within a given window/DMR.
