Chunk #8 — Methods — Neural stem cell isolation, culture and differentiation

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Decitabine alters the expression of Mecp2 isoforms via dynamic DNA methylation at the Mecp2 regulatory elements in neural stem cells.

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Mississauga, Ontario, Canada) at a density of 105 cells/cm2 in DMEM (GIBCO, Life Technologies Inc, Burlington, Ontario, Canada) and 10% Fetal Bovine Serum (Invitrogen, Life Technologies Inc, Burlington, Ontario, Canada) in the absence of rhEGF and bFGF. Cells were differentiated for 8 days, reported to be sufficient for differentiation of neuronal and glial cells [35,37], and media were changed every other day.