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Chunk #21 — Methods — Predictions of mRNA secondary structure

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Association between KCNJ6 (GIRK2) gene polymorphisms and postoperative analgesic requirements after major abdominal surgery.
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To discuss in depth mRNA sequence and function, the secondary structure for KCNJ6 mRNA was predicted using Mfold web server (v. 3.2) with default settings [25]. The KCNJ6 mRNA position 982–1082 was used to predict the local structure of the mRNA based on the nucleotide sequences of the GenBank database (accession number: NM_002240.2).