To evaluate representations at the level of cell class, we performed two sets of stereological counting experiments using IHC and transgenic mouse lines. In the first experiment, we used the Ald1l1-GFP (Jackson Labs, 026033) line to drive GFP expression in astrocytes and the immuno-labeled Neurons using anti-NeuN (Millipore, MAB377) antibody and Oligodendrocyte/Polydendrocyte using anti-Olig2 (Millipore, AB9610) antibody. We visualized NeuN using a secondary antibody in the red channel (goat-anti mouse 568; Abcam, ab175473) and Olig2 (goat-anti Rabbit 647; Abcam, ab150079) in far-red. GFP was not immune-enhanced. Sections were mounted in media containing DAPI as counterstain (Vector Laboratories, Vectashield). Four sections containing Frontal Cortex were counted from n=1 mouse, using n=34 random fields with n=9 boxes sampled from each. In the second set of experiments, we replaced Ald1l1-GFP with Cx3cr1-GFP mice (Jackson Labs, 005582) to label microglia instead of astrocytes. Three sections were counted from n=2 mice, using n=68 random fields in total with n=9 boxes sampled from each.
