Cell suspensions were generated from adult male mice (60–70 days old; C57Blk6/N). Two BAC transgenic mouse lines Ald1l1-EGFP (Jackson Labs, 026033) and Cx3cr1-GFP (Jackson Labs, 005582) used in IHC experiments were also included as single replicates in the Frontal Cortex Drop-seq dataset. Both transgenics were on a C57BL/6N background. To estimate the fraction of cells retained in single-cell suspensions, Nestin-Cre driver mice (Jackson Labs, 003771) were bred lox-stop-lox (lsl) ZsGreen Cre-reporter (Jackson Labs, 007906). Mice were group housed prior to experimentation on a reverse 12-hour light-dark schedule. All experiments were approved by and in accordance with Harvard Medical School IACUC protocol number IS00000055–3.
