Selected human enhancers (CRE1-5) were amplified from human genomic DNA using primers (Supplementary Table 9). PCR products were purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey Nagel) and were digested using appropriate enzymes (listed in Supplementary Table 9). Human enhancers were cloned into EcoRV/SpeI or HindIII/EcoRI sites of pDB896 vector (gift from Darius Balciunas) upstream of zebrafish gata2 promoter63,64 and YFP reporter gene. Plasmid DNA was purified using NucleoBond® Xtra Midi Kit (Macherey Nagel) and quality checked by sequencing before injections.
