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Chunk #6 — MATERIALS AND METHODS — Selection of SDPS‐prone vs SDPS‐resilient groups

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Stress vulnerability promotes an alcohol-prone phenotype in a preclinical model of sustained depression.
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SDPS rats were assigned to either SDPS‐prone or SDPS‐resilient subgroups following a two‐step cluster analysis of affective and cognitive performance using Schwarz's Bayesian criterion.16 In particular, rats were clustered based on their individual scores in the social approach‐avoidance (SAA) and in object place recognition (OPR) tasks, assessed in weeks 5 and 9 postdefeat (for details, see Figure S1 and the study of Riga, Schmitz, Hoogendijk, Smit, and Spijker21). From the emerging SDPS‐prone and SDPS‐resilient groups, data obtained from 10 rats (n = 5 per subgroup) were used to describe alcohol‐related effects of SDPS in the general population,20 thus, were not included in the alcohol SA analysis presented here. Control animals were divided in two equally performing groups (balanced average performance in SAA and OPR tests), and a total of 16 control rats participated in the experiments described below.