Chunk #36 — Anticipated Results

Source

Efficient, footprint-free human iPSC genome editing by consolidation of Cas9/CRISPR and piggyBac technologies.

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The first stage of this protocol is to integrate the piggyBac transposon containing the Dox-inducible Cas9 expression cassette into iPSCs, to yield iPSC-Cas9-PB. This step only needs to be done once to obtain a common parental line that is conducive for genome editing.