AUD high-PRS subjects that the high-PRS iPSCs were derived from carried certain epigenetic changes that confer the differences between gene expression and functional differences between the high-PRS versus low-PRS microglial cells. How AUD and PRS may interact with histone acetylation, as affected by ethanol metabolites and open chromatin state to influence gene expression in vivo and in vitro, needs to be further elucidated.
