When, first, all 40 variables were forced to enter DFA on this population of 137 subjects (C-group, n = 22; ASD-group, n = 115), despite the unequal subject number per group, a highly significant group differentiation was again observed by Wilks' Lambda (0.155), with Rao's approximation (F = 13.09; df = 40,96; P < 0.0001). The C-group subjects and the ASD-group patients were both classified with 100% accuracy.