Chunk #32 — Experimental Procedures — Gibson Assembly of Vectors

Source

Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes.

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yes

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The following vectors have been deposited at Addgene: lenti-EF1a-dCas9-VP64-Puro, lenti-EF1a-dCas9-VPR-Puro, lenti-EF1a-dCas9-KRAB-Puro, lentiGuide-Hygro-mTagBFP2, lentiGuide-Hygro-eGFP, lentiGuide-Hygro-dTomato, lentiGuide-Hygro-iRFP670, and pLV-TetO-hNGN2-Neo.