To assess motor coordination, WT and KO mice (n = 10) were trained to remain on a rotating rod apparatus (Rotarod; Ugo Basile, Comerio, Italy), revolving at 16 revolutions per minute. Each animal had two 5 minute training sessions. The following day, all animals were re-tested for their ability to complete a 3 minute trial prior to drug administration. Animals were injected with 2 g/kg ethanol and tested on the Rotarod at 20 minute intervals until two consecutive 3 minute trials were completed without falling.
