Ethanol alters mitochondrial patterns in iPS cells, NPCs, and NPC-derived neurons. Confocal microscopy images showing the mitochondrial pattern (Mitotracker) in iPS cells (a), NPCs (b), and NPC-derived neurons (after 26 days of differentiation) (c) after treatment with ethanol for 24hr or 7d. Nuclei (in blue) are counterstained with DAPI. Inserts: co-immunolabeling with MAP2 shows different colocalization of Mitotracker in neuronal cells. Scale bars: 10 μm