loss of heterozygosity (LOH) occurs in a cell-specific manner in TSC (Crino, 2013) and it is possible that LOH in Purkinje cells accounts for some of the ASD symptoms in human TSC patients, but more work needs to be done to confirm this hypothesis. More recently, a group knocked out Tsc1 in all excitatory forebrain neurons (CaMKIIα-Cre) and selectively in serotonergic neurons (Slc6a4-Cre) and each was sufficient to produce ASD-like behaviors (McMahon et al., 2014).
