only in a 1:1 ratio (10 ng:10 ng); 2) α2β2γ3 in a 1:1:3 ratio (10 ng:10 ng:30 ng); 3) α2β2γ3 in a 1:1:1 ratio (10 ng:10 ng:10 ng); 4) α2β2γ3 in a 3:1:1 ratio (30 ng:10 ng:10 ng); 5) α2β2γ3 in a 2:2:1 ratio (10 ng:10 ng:5 ng); 6) α2β2γ3 in a 6:2:1 (30 ng:10 ng:5 ng); 7) α2β2γ3 in a 0.5:2:1 ratio (5 ng:20 ng:10 ng). Since the transcripts and coding regions for all 3 genes are similar in length the molarities will also be similar when 1:1:1 quantities of RNA are injected. Because all 3 genes have optimal Kozak sequences, the ratios of injected subunit mRNAs should be reflected in the amount of specific subunit protein expressed. Oocytes were stored in Oocyte Ringer solution (50% Leibovitz’s L15 medium, 15 mM HEPES, 0.8 mM L-glutamine, 0.04 mg/mL gentamycin) at 17° C.
