the behavioral effects over this time course is likely to be due to compensatory mechanisms that act in the opposite direction to ethanol or directly limit the action of ethanol. Second, ADH mutant animals develop acute tolerance similar to that displayed by wild-type animals showing that a reduction in metabolism does not impact the mechanisms of acute tolerance development (Figure 3a). This provides further support for the idea that acute tolerance is occurring at the level of the affected tissues, which in C. elegans includes the nervous system (Davies et al., 2003) rather than through a systemic metabolic process.
