Chunk #4 — MATERIALS AND METHODS — Antibody information and chemicals

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Endogenous GluR1-containing AMPA receptors translocate to asymmetric synapses in the lateral amygdala during the early phase of fear memory formation: an electron microscopic immunocytochemical study.

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Chemicals required for the osmium-free processing of tissue for EM (Phend et al., 1995) included glutaraldehyde, paraformaldehyde, tannic acid, p-phenylenediamine (PPND), uranyl acetate, and EMBED-812. These were purchased from EMS. Iridium tetrabromide (IrB4), another chemical required for osmium-free tissue processing, was purchased from Premion (Wardhill, MA, cat. no. 39493). Maleate buffer salts, Tris buffer salts, and Triton X-100 were purchased from Sigma (St. Louis, MO).