Generalized linear models were used to test for sex and strain main effects, and sex × strain interaction effects (Table 3, Supplementary information, Table S1). False discovery rate analysis was used to control the family-wise error rate at 0.05 and showed significant sex effects for 97 of the 257 measures with five expected false positives, significant strain effects for all 257 measures, and significant sex × strain interactions for 144 of the 257 measures with seven expected false positives. In the event that there was no trend toward a significant sex difference or interaction, male and female data were combined for subsequent QTL mapping and genetic correlations.
