Electrophysiological recordings were obtained from neurons from subject 1 (a non-alcoholic) using previously described techniques (Lemtiri-Chlieh and Levine, 2010). Neurons were selected for recording based on neural morphology (neurite projections and pyramidal-shaped soma). Artificial cerebrospinal fluid (aCSF) containing 125mM NaCl, 2.5mM KCl, 1.25mM NaH2PO4, 1mM MgCl2-6H2O, 25mM NaHCO3, 2mM CaCl2, and 25mM dextrose was perfused into the recording chamber at 1mL/min at room temperature. To examine NMDA responses to locally applied glutamate, the bath solution contained the AMPA receptor antagonist DNQX (10µM) under Mg2+ free conditions to relieve the voltage-dependent Mg2+ block of NMDA receptors.
