aCGH was conducted using a custom 4 × 180 K microarray (Agilent Technologies); genotypes were confirmed by long-range PCR. Karyotyping and FISH were performed by Wicell Cytogenetics (Madison, WI). See Supplemental Experimental Procedures for detailed descriptions of aCGH, karyotype, FISH, PCR, DNA fingerprinting, and immunocytochemical techniques.
