While our electrophysiological recordings clearly demonstrate the presence of functional GABAA receptors, it may be that robust endogenous GABAergic synaptic transmission is needed during the alcohol exposure period to observe compensatory changes in expression and function. Utilizing a differentiation protocol to produce a heterogeneous population of glutamatergic and GABAergic neurons, or co-culturing enriched cultures, may provide a model system more relevant to the human brain.
